Journal: PLoS ONE
Article Title: Pre-Osteoblasts Stimulate Migration of Breast Cancer Cells via the HGF/MET Pathway
doi: 10.1371/journal.pone.0150507
Figure Lengend Snippet: (a) Low HGF gene levels in HS27A-derived pre-OBs . mRNA expression of HGF was analyzed by quantitative PCR and expressed as fold increase of undifferentiated cells. (b) CM of HS27A-derived pre-OBs do not induce BC cell migration . Wound healing assay was performed to assess migration of the MDA-MB231 BC cell line in the presence of CM from HS27A-derived osteolineage cells. Results are expressed as percentage of negative control.
Article Snippet: The human bone marrow stromal cell line (BMSCs) HS27A was purchased from the American Type Culture Collection (ATCC [ ]), KM105 cells were a kind gift of Dr. Kenichi (Chiba University Graduate School of Medicine, Chiba, Japan) [ ].
Techniques: Derivative Assay, Expressing, Real-time Polymerase Chain Reaction, Migration, Wound Healing Assay, Negative Control